0428 GMT May 23, 2018
Yet investigators in the Division of Infectious Diseases at the University of North Carolina (UNC) School of Medicine have found the virus persists in HIV-infected macrophages, according to medicalxpress.com.
Macrophages are large white blood cells found in tissues throughout the body including the liver, lung, bone marrow and brain.
The discovery of this additional viral reservoir has significant implications for HIV cure research. These findings were published in Nature Medicine.
Jenna Honeycutt, PhD, lead-author and postdoctoral research associate in the UNC Division of Infectious Diseases, said, "These results are paradigm changing because they demonstrate that cells other than T cells can serve as a reservoir for HIV.
"The fact that HIV-infected macrophages can persist means that any possible therapeutic intervention to eradicate HIV might have to target two very different types of cells."
Last spring, this laboratory lead by J. Victor Garcia, PhD, professor of medicine, microbiology and immunology at UNC School of Medicine, demonstrated the ability of tissue macrophages to support HIV replication in vivo in the total absence of human T cells.
But how macrophages would respond to antiretroviral therapy (ART) and whether macrophages represented a reservoir for HIV after treatment were unknown.
Macrophages are myeloid lineage cells that have been implicated in HIV pathogenesis and in the trafficking of virus into the brain.
Using a humanized myeloid-only mouse (MoM) model devoid of T cells, Garcia and his team showed that ART strongly suppresses HIV replication in tissue macrophages.
Yet when HIV treatment was interrupted, viral rebound was observed in one third of the animals. This is consistent with the establishment of persistent infection in tissue macrophages.
Honeycutt said, "This is the first report demonstrating that tissue macrophages can be infected and that they respond to antiretroviral therapy.
"In addition, we show that productively infected macrophages can persist despite ART; and most importantly, that they can reinitiate and sustain infection upon therapy interruption even in the absence of T cells — the major target of HIV infection."
Now that Garcia and his team know HIV persists in macrophages, the next step will be to determine what regulates HIV persistence in tissue macrophages, where in the body persistently infected macrophages reside during HIV treatment and how macrophages respond to possible therapeutic interventions aimed at eradicating HIV from the body.