March 29, 2017 0318 GMT
In a statement Tuesday, the Turkish Foreign Ministry said the EU's stance on Turkey was "shortsighted" and "carried no value" for Turkey, as well as lending "credence" to extremists, AP reported.
The spat has raised concerns that cooperation between the EU and Turkey on a number of issues, such as dealing with the flow of migrants from war-torn Syria, may start to fray.
The ministry argued that the European bloc had "ignored the (Netherlands') violation of diplomatic conventions and the law" after Dutch authorities escorted the Turkish family affairs minister out of the country and denied the foreign minister permission to land.
The two ministers had sought to campaign and court the votes of Turks in the Netherlands that are eligible to vote in an April 16 referendum on expanding Turkish President Recep Tayyip Erdogan's powers. Around 400,000 people with ties to Turkey live in the Netherlands.
The diplomatic spat between the two countries escalated swiftly with Erdogan making several Nazi comparisons with EU member states Germany and the Netherlands. The EU has called on Turkey to cease "excessive statements."
On Monday, Turkey slapped a series of political sanctions against the Netherlands, including halting political discussions between the two countries and closing Turkish airspace to Dutch diplomats. Other sanctions bar the Dutch ambassador entry back into Turkey and advise parliament to withdraw from a Dutch-Turkish friendship group.
Deputy Prime Minister Numan Kurtulmus said the sanctions would apply until the Netherlands takes steps "to redress" the actions that Ankara sees as a grave insult.
German Chancellor Angela Merkel also backed the Netherlands in its diplomatic fight with Turkey, pledging her full support and solidarity with the Dutch and saying the Nazi gibes were unacceptable.
Erdogan responded angrily to Merkel's support for the Netherlands, exclaiming "Shame on you!" during a television interview on Monday.
Merkel has refrained from reacting to Erdogan.
"The chancellor has no intention of participating in the race of provocations," Merkel's spokesman, Steffen Seibert, said late Monday. "She won't play along. The accusations are recognizably absurd."
Also Monday, the German Foreign Ministry amended its travel advice for Turkey, noting that "elevated political tensions and protests that could also be directed against Germany" should be expected during the referendum campaign. It recommended that travelers stay away from political events and large gatherings of people.